[PDF][PDF] Analysis of tRNA population from Drosophila melanogaster by means of polyacrylamide gel mapping

A Mazabraud, JP Garel - FEBS letters, 1979 - core.ac.uk
Two-dimensional polyacrylamide gel electrophoresis of tRNA [l] is becoming a remarkable
tool to investigate various problems dealing with the regulation of protein synthesis: accurate
quantitation of mature tRNA species [2-41; purification of individual tRNA species [3, 5, 6];
identification and quantitation of tRNA precursors [4, 7] and maturation products [8]; turnover
rates [4]; distribution and arrangement of tRNA genes [4, 9, 11]; test of purity of [1251]
iodotRNA [12, 13]. In each case, however, the tRNA spots on the gel map must be identified …
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